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KMID : 1007520120210030917
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Food Science and Biotechnology
2012 Volume.21 No. 3 p.917 ~ p.920
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In vivo Imaging of Escherichia coli and Lactococcus lactis in Murine Intestines Using a Reporter Luciferase Gene
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Lee Mi-Sook
Moon Gi-Seong
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Abstract
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A recombinant DNA, pMG36e::luc+ (5.3 kb), was constructed and transferred to Escherichia coli MC1061 and Lactococcus lactis MG1363 for in vivo imaging of the bacteria in murine intestines. E. coli MC1061 (pMG36e::luc+) and L. lactis MG1363 (pMG36e::luc+) displayed luciferase activities in vitro, where the bioluminescent signal of the E. coli was much stronger than that of the L. lactis by approximately 100- fold. These 2 recombinant bacteria were orally administered into rats. The bioluminescent signals of the E. coli and L. lactis in the gastrointestinal (GI) tracts of rats were detected and maintained up to 2 h via whole body imaging, indicating that the luciferase gene expression system for bacteria could be applied for in vivo imaging.
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KEYWORD
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in vivo imaging, Lactococcus lactis, luciferase, pMG36e, recombinant DNA
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